6LVS

USP14 catalytic domain mutant C114S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Ubiquitin carboxyl-terminal hydrolase 14	polymer	410	46982.3	6	UniProt (P54578) Pfam (PF00443)	Homo sapiens (Human)	Deubiquitinating enzyme 14,Ubiquitin thioesterase 14,Ubiquitin-specific-processing protease 14
2	FB, G, GB, H, I... (D, A, C, E, B)	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
3	AA, BA, CA, DA, GC... (B, F, D, A, C...)	SODIUM ION	non-polymer		23.0	41	Chemie (NA)
4	AB, AC, BB, BC, CB... (C, E, B, D, F...)	FORMIC ACID	non-polymer		46.0	26	Chemie (FMT)
5	CC, DB, DC, EB, EC... (E, C, B, F, A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	9	Chemie (BME)
6	FC (E)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	RC, SC, TC, UC, VC... (A, B, C, D, E...)	water	water		18.0	354	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 92 - 494 (UniProt: P54578)

PDB	External Database	Details
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
Ser 82	-	expression tag
Ser 83	-	expression tag
Gly 84	-	expression tag
Leu 85	-	expression tag
Val 86	-	expression tag
Pro 87	-	expression tag
Arg 88	-	expression tag
Gly 89	-	expression tag
Ser 90	-	expression tag
His 91	-	expression tag
Ser 114	Cys 114	engineered mutation
-	Val 223	deletion
-	Lys 224	deletion
-	Glu 225	deletion
-	Thr 226	deletion
-	Asp 227	deletion
-	Ser 228	deletion
-	Ser 229	deletion
-	Ser 230	deletion
-	Ala 231	deletion

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	281893.5
Non-Polymers*	Number of molecules	89
	Total formula weight	4009.6
All*	Total formula weight	285903.2

*Water molecules are not included.