6LTV

Crystal Structure of I122A/I330A variant of S-adenosylmethionine synthetase from Cryptosporidium hominis in complex with ONB-SAM (2-nitro benzyme S-adenosyl-methionine)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	S-adenosylmethionine synthase	polymer	414	45871.2	2	UniProt (A0A0S4TKQ5) Pfam (PF00438) Pfam (PF02772) Pfam (PF02773)	Cryptosporidium hominis
2	C, F (A, B)	TRIPHOSPHATE	non-polymer		258.0	2	Chemie (3PO)
3	D, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, H (A, B)	[(2S,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl-[(3R)-3-azanyl-4-oxidanyl-4-oxidanylidene-butyl]-[(2-nitrophenyl)methyl]sulfanium	non-polymer		520.5	2	Chemie (EU9)
5	I, J (A, B)	water	water		18.0	367	Chemie (HOH)

Sequence modifications

A, B: 1 - 406 (UniProt: A0A0S4TKQ5)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 122	Ile 122	variant
Ala 330	Ile 330	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91742.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1605.6
All*	Total formula weight	93347.9

*Water molecules are not included.