6LPB

Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	P	Pituitary adenylate cyclase-activating polypeptide	polymer	38	4547.3	1	UniProt (P18509) Pfam (PF00123) In PDB	Homo sapiens	PACAP
2	R	Pituitary adenylate cyclase-activating polypeptide type I receptor	polymer	403	46675.5	1	UniProt (P41586) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	PACAP-R1
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
4	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	249	28964.7	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens
5	N	nanobody Nb35	polymer	137	15015.7	1		unidentified
6	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I

Sequence modifications

R: 21 - 417 (UniProt: P41586)

PDB	External Database	Details
Glu 418	-	expression tag
Asn 419	-	expression tag
Leu 420	-	expression tag
Tyr 421	-	expression tag
Phe 422	-	expression tag
Gln 423	-	expression tag

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	141495.4
All*	Total formula weight	141495.4

*Water molecules are not included.