6LI2

Crystal structure of GPR52 ligand free form with rubredoxin fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Chimera of G-protein coupled receptor 52 and Rubredoxin	polymer	372	42510.4	1	UniProt (Q9Y2T5) UniProt (P00268) Pfam (PF00001) Pfam (PF00301)	Homo sapiens (Human)	Rd
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C, D, E, F, G... (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	11	Chemie (OLC)
4	N (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	O (A)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 17 - 235 (UniProt: Q9Y2T5)

PDB	External Database	Details
Gly 16	-	expression tag
Trp 130	Ala 130	conflict

A: 1002 - 1054 (UniProt: P00268)

PDB	External Database	Details
Tyr 263	-	linker
Leu 264	-	linker

A: 265 - 340 (UniProt: Q9Y2T5)

PDB	External Database	Details
Met 1001	-	linker
Gln 278	Trp 278	engineered mutation
Pro 314	Cys 314	engineered mutation
Ala 318	Ser 318	engineered mutation
Asp 321	Asn 321	engineered mutation
Thr 323	Val 323	engineered mutation
Glu 601	-	expression tag
Phe 602	-	expression tag
Leu 603	-	expression tag
Glu 604	-	expression tag
Val 605	-	expression tag
Leu 606	-	expression tag
Phe 607	-	expression tag
Gln 608	-	expression tag
Gly 609	-	expression tag
Pro 610	-	expression tag
His 611	-	expression tag
His 612	-	expression tag
His 613	-	expression tag
His 614	-	expression tag
His 615	-	expression tag
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42510.4
Non-Polymers*	Number of molecules	13
	Total formula weight	4093.5
All*	Total formula weight	46603.9

*Water molecules are not included.