6LGI

Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate complexed with fructose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Sucrose hydrolase	polymer	598	68738.2	2	UniProt (A0A077JI83) Pfam (PF00128) In PDB	Bombyx mori (Silk moth)
2	A, B	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A, B	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
5	A, B	beta-D-fructofuranose	non-polymer		180.2	2	Chemie (FRU)
6	A, B	beta-D-fructopyranose	non-polymer		180.2	2	Chemie (BDF)
7	A, B	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
8		water	water		18.0	983	Chemie (HOH)

Sequence modifications

A, B: 30 - 606 (UniProt: A0A077JI83)

PDB	External Database	Details
Met 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
His 28	-	expression tag
Met 29	-	expression tag
Gln 322	Glu 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	137476.4
Non-Polymers*	Number of molecules	15
	Total formula weight	1400.7
All*	Total formula weight	138877.1

*Water molecules are not included.