6LDT

K245A mutant of L-tyrosine decarboxylase from Methanocaldococcus jannaschii complexed with a post-decarboxylation quinonoid-like intermediate formed with L-tyrosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	L-tyrosine/L-aspartate decarboxylase	polymer	415	47600.7	1	UniProt (Q60358) Pfam (PF00282)	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	TDC/ADC
2	B, C, D (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	E (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	F (A)	[(4Z)-4-[[(Z)-2-(4-hydroxyphenyl)ethylideneamino]methylidene]-6-methyl-5-oxidanyl-1H-pyridin-3-yl]methyl dihydrogen phosphate	non-polymer		366.3	1	Chemie (EBR)
5	G (A)	water	water		18.0	273	Chemie (HOH)

Sequence modifications

A: 1 - 396 (UniProt: Q60358)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Asp -15	-	expression tag
Lys -14	-	expression tag
Ile -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 245	Lys 245	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47600.7
Non-Polymers*	Number of molecules	5
	Total formula weight	738.7
All*	Total formula weight	48339.4

*Water molecules are not included.