6LDR

Structure of a K245A mutant of a Group II PLP dependent decarboxylase from Methanocaldococcus jannaschii, in complex with PLP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	L-tyrosine/L-aspartate decarboxylase	polymer	415	47600.7	1	UniProt (Q60358) Pfam (PF00282) In PDB	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	TDC/ADC
2	A	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	1	Chemie (PLP)
3	A	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
4	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6		water	water		18.0	326	Chemie (HOH)

Sequence modifications

A: 1 - 396 (UniProt: Q60358)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Asp -15	-	expression tag
Lys -14	-	expression tag
Ile -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 245	Lys 245	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47600.7
Non-Polymers*	Number of molecules	5
	Total formula weight	545.4
All*	Total formula weight	48146.2

*Water molecules are not included.