6LB8

Crystal structure of the Ca2+-free T4L-MICU1-MICU2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Endolysin,Calcium uptake protein 1, mitochondrial	polymer	527	60321.7	2	UniProt (D9IEF7) UniProt (Q9BPX6) Pfam (PF00959) Pfam (PF13202)	Escherichia virus T4	Lysis protein,Lysozyme,Muramidase,Atopy-related autoantigen CALC,ara CALC,Calcium-binding atopy-related autoantigen 1
2	B, D (B, D)	Calcium uptake protein 2, mitochondrial	polymer	330	38719.2	2	UniProt (Q8IYU8)	Homo sapiens (Human)	EF-hand domain-containing family member A1

Sequence modifications

A, C: 1001 - 1161 (UniProt: D9IEF7)

PDB	External Database	Details
Met 993	-	expression tag
Gly 994	-	expression tag
His 995	-	expression tag
His 996	-	expression tag
His 997	-	expression tag
His 998	-	expression tag
His 999	-	expression tag
His 1000	-	expression tag
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
His 1162	-	linker
Met 1163	-	linker

A, C: 97 - 444 (UniProt: Q9BPX6)

PDB	External Database	Details
Gly 445	-	expression tag
Ser 446	-	expression tag
Gly 447	-	expression tag
Ser 448	-	expression tag
Gly 449	-	expression tag
Ser 450	-	expression tag
Gly 451	-	expression tag
Ser 452	-	expression tag

B, D: 84 - 406 (UniProt: Q8IYU8)

PDB	External Database	Details
Gly 77	-	expression tag
Ser 78	-	expression tag
Gly 79	-	expression tag
Ser 80	-	expression tag
Gly 81	-	expression tag
Ser 82	-	expression tag
Gly 83	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198081.9
All*	Total formula weight	198081.9

*Water molecules are not included.