6L4V

Turning an asparaginyl endopeptidase into a peptide ligase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Asparaginyl endopeptidase	polymer	502	55773.9	1	UniProt (A0A0P0QM28) Pfam (PF01650) Pfam (PF20985) In PDB	Clitoria ternatea (Butterfly pea)	Butelase 2A
2	A	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	536	Chemie (HOH)

Sequence modifications

A: 33 - 488 (UniProt: A0A0P0QM28)

PDB	External Database	Details
Met -13	-	expression tag
Val -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ile -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Leu -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
His 2	-	expression tag
Ser 3	-	expression tag
Ala 4	-	expression tag
Phe 5	-	expression tag
Ala 6	-	expression tag
Ala 7	-	expression tag
Ala 8	-	expression tag
Met 9	-	expression tag
Gly 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Val 20	-	expression tag
Asp 21	-	expression tag
Leu 22	-	expression tag
Gly 23	-	expression tag
Thr 24	-	expression tag
Glu 25	-	expression tag
Asn 26	-	expression tag
Leu 27	-	expression tag
Tyr 28	-	expression tag
Phe 29	-	expression tag
Gln 30	-	expression tag
Ser 31	-	expression tag
Met 32	-	expression tag
Snn 179	Asp 179	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55773.9
Non-Polymers*	Number of molecules	6
	Total formula weight	416.5
All*	Total formula weight	56190.3

*Water molecules are not included.