6L18
XFEL structure of T4dCH D179N mutant complex with natively expressed dTMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Deoxycytidylate 5-hydroxymethyltransferase | polymer | 256 | 29869.7 | 1 | UniProt (P08773) Pfam (PF00303) | Enterobacteria phage T4 | Deoxycytidylate hydroxymethylase,dCMP hydroxymethylase,dCMP HMase |
| 2 | B (A) | THYMIDINE-5'-PHOSPHATE | non-polymer | 322.2 | 1 | Chemie (TMP) | |||
| 3 | C (A) | IODIDE ION | non-polymer | 126.9 | 1 | Chemie (IOD) | |||
| 4 | D (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | E (A) | water | water | 18.0 | 60 | Chemie (HOH) |
Sequence modifications
A: 1 - 246 (UniProt: P08773)
| PDB | External Database | Details |
|---|---|---|
| Asn 179 | Asp 179 | engineered mutation |
| Leu 247 | - | expression tag |
| Glu 248 | - | expression tag |
| His 249 | - | expression tag |
| His 250 | - | expression tag |
| His 251 | - | expression tag |
| His 252 | - | expression tag |
| His 253 | - | expression tag |
| His 254 | - | expression tag |
| His 255 | - | expression tag |
| His 256 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 29869.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 472.1 | |
| All* | Total formula weight | 30341.8 |
