6L18

XFEL structure of T4dCH D179N mutant complex with natively expressed dTMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Deoxycytidylate 5-hydroxymethyltransferase	polymer	256	29869.7	1	UniProt (P08773) Pfam (PF00303) In PDB	Enterobacteria phage T4	Deoxycytidylate hydroxymethylase,dCMP hydroxymethylase,dCMP HMase
2	A	THYMIDINE-5'-PHOSPHATE	non-polymer		322.2	1	Chemie (TMP)
3	A	IODIDE ION	non-polymer		126.9	1	Chemie (IOD)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5		water	water		18.0	60	Chemie (HOH)

Sequence modifications

A: 1 - 246 (UniProt: P08773)

PDB	External Database	Details
Asn 179	Asp 179	engineered mutation
Leu 247	-	expression tag
Glu 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29869.7
Non-Polymers*	Number of molecules	3
	Total formula weight	472.1
All*	Total formula weight	30341.8

*Water molecules are not included.