6L18
XFEL structure of T4dCH D179N mutant complex with natively expressed dTMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | PAL-XFEL BEAMLINE NCI |
| Synchrotron site | PAL-XFEL |
| Beamline | NCI |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2018-09-17 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.2782 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.860, 74.970, 157.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.492 - 1.900 |
| Rwork | 0.169 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5e |
| Data reduction software | XDS |
| Data scaling software | CrystFEL |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 27244 | 2687 |
| <I/σ(I)> | 4.87 | 2.51 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 595.9 | 176.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 8.5 | 293 | 2 ul of 1.0 M Tris-HCl pH 8.5 2 ul of 1.0 M NaI 17 ul of C6H5Na3 2H2O 10 ul of 40mg/ml D179N mutant protein |
