6KOY

Crystal structure of two domain M1 Zinc metallopeptidase E323A mutant bound to L-tryptophan amino acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Zinc metalloprotease	polymer	474	51547.8	2	UniProt (Q9RVZ5) Pfam (PF17900) Pfam (PF01433) In PDB	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	M1 zinc metallopeptidase
2	A, B	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	332	Chemie (HOH)

Sequence modifications

A, B: 36 - 472 (UniProt: Q9RVZ5)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag
Ser 1	-	expression tag
Trp 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
Pro 5	-	expression tag
Gln 6	-	expression tag
Phe 7	-	expression tag
Glu 8	-	expression tag
Lys 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Gly 23	-	expression tag
Gly 24	-	expression tag
Gly 25	-	expression tag
Gly 26	-	expression tag
Gly 27	-	expression tag
Glu 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Tyr 31	-	expression tag
Phe 32	-	expression tag
Gln 33	-	expression tag
Gly 34	-	expression tag
Ser 35	-	expression tag
Ala 323	Glu 323	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	103095.6
Non-Polymers*	Number of molecules	4
	Total formula weight	539.3
All*	Total formula weight	103634.9

*Water molecules are not included.