6KJ0

Bifunctional xylosidase/glucosidase LXYL mutant E529Q C2221

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-D-xylosidase/beta-D-glucosidase	polymer	809	86864.7	2	UniProt (G8GLP2)	Lentinula edodes (Shiitake mushroom)
2	C, F (C, F)	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G62182OO)
3	D, G (D, G)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	2	In PDB GlyTouCan (G07617FP)
4	E, H (E, H)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
5	I, J, K, L, O... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)
6	M, S (A, B)	Deacetyltaxol	non-polymer		811.9	2	Chemie (BKR)
7	N, T (A, B)	beta-D-xylopyranose	non-polymer		150.1	2	Chemie (XYP)
8	U, V (A, B)	water	water		18.0	680	Chemie (HOH)

Sequence modifications

A, B: 1 - 803 (UniProt: G8GLP2)

PDB	External Database	Details
Gln 529	Glu 529	engineered mutation
His 804	-	expression tag
His 805	-	expression tag
His 806	-	expression tag
His 807	-	expression tag
His 808	-	expression tag
His 809	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	173729.3
Branched	Number of molecules	6
	Total formula weight	5140.7
Non-Polymers*	Number of molecules	12
	Total formula weight	3693.7
All*	Total formula weight	182563.6

*Water molecules are not included.