6KJ0
Bifunctional xylosidase/glucosidase LXYL mutant E529Q C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.998, 182.228, 241.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 120.760 - 2.270 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.760 | 2.300 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.122 | 0.548 |
| Number of reflections | 76271 | 3798 |
| <I/σ(I)> | 27.1 | |
| Completeness [%] | 93.1 | 94.1 |
| Redundancy | 8.6 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 15% PEG 3350, 0.1M Hepes pH 7.5, 0.2% MgCl2 |
