6KI0

Crystal Structure of Human ASC-CARD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Maltose/maltodextrin-binding periplasmic protein,Apoptosis-associated speck-like protein containing a CARD	polymer	466	51490.3	2	UniProt (P0AEX9) UniProt (Q9ULZ3) Pfam (PF01547) Pfam (PF00619)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,hASC,Caspase recruitment domain-containing protein 5,PYD and CARD domain-containing protein,Target of methylation-induced silencing 1
2	C, D (C, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
3	E, F, G (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	H, I (A, B)	water	water		18.0	269	Chemie (HOH)

Sequence modifications

A, B: 1 - 358 (UniProt: P0AEX9)

PDB	External Database	Details
Met 0	-	expression tag
Ala 82	Asp 108	engineered mutation
Ala 83	Lys 109	engineered mutation
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 239	Lys 265	engineered mutation
Ala 359	-	linker
Ala 360	-	linker
Leu 361	-	linker
Ala 362	-	linker
Ala 363	-	linker
Ala 364	-	linker
Gln 365	-	linker
Thr 366	-	linker
Asn 367	-	linker
Ala 368	-	linker
Val 369	-	linker
Asp 370	-	linker

A, B: 371 - 454 (UniProt: Q9ULZ3)

PDB	External Database	Details
Ala 455	-	expression tag
Ala 456	-	expression tag
Ala 457	-	expression tag
Leu 458	-	expression tag
Glu 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102980.5
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	104277.6

*Water molecules are not included.