6KF4
Cryo-EM structure of Thermococcus kodakarensis RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 906 | 103038.6 | 1 | UniProt (Q5JE33) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1123 | 127468.0 | 1 | UniProt (Q5JE32) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 3 | C (C) | DNA-directed RNA polymerase subunit A'' | polymer | 391 | 43727.4 | 1 | UniProt (Q5JE34) Pfam (PF04998) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 4 | D (D) | DNA-directed RNA polymerase subunit D | polymer | 259 | 29429.6 | 1 | UniProt (Q5JJF4) Pfam (PF01193) Pfam (PF01000) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 5 | E (E) | DNA-directed RNA polymerase, subunit E | polymer | 190 | 21893.4 | 1 | UniProt (Q5JIY4) Pfam (PF03876) Pfam (PF00575) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 6 | F (F) | DNA-directed RNA polymerase, subunit F | polymer | 122 | 14519.7 | 1 | UniProt (Q5JI52) Pfam (PF03874) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 7 | G (H) | DNA-directed RNA polymerase subunit H | polymer | 82 | 9522.0 | 1 | UniProt (Q5JE31) Pfam (PF01191) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 8 | H (K) | DNA-directed RNA polymerase subunit K | polymer | 57 | 6286.5 | 1 | UniProt (Q5JJD0) Pfam (PF01192) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 9 | I (L) | DNA-directed RNA polymerase subunit L | polymer | 94 | 11013.5 | 1 | UniProt (Q5JE88) Pfam (PF13656) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 10 | J (N) | DNA-directed RNA polymerase subunit N | polymer | 65 | 7602.0 | 1 | UniProt (Q5JJC9) Pfam (PF01194) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 11 | K (P) | DNA-directed RNA polymerase subunit P | polymer | 49 | 5553.7 | 1 | UniProt (Q5JDM8) Pfam (PF03604) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | |
| 12 | L (G) | Transcription factor E | polymer | 185 | 22088.6 | 1 | UniProt (Q5JDD5) Pfam (PF02002) | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) | TFE,TFIIE subunit alpha homolog,Transcription initiation factor TFIIE |
| 13 | M (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 14 | N, O, P, Q, R... (A, B, N, P, G) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence modifications
F: 1 - 114 (UniProt: Q5JI52)
| PDB | External Database | Details |
|---|---|---|
| Ile 115 | - | expression tag |
| Asp 116 | - | expression tag |
| Glu 117 | - | expression tag |
| Tyr 118 | - | expression tag |
| Arg 119 | - | expression tag |
| Pro 120 | - | expression tag |
| Leu 121 | - | expression tag |
| Glu 122 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 402143.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 416.8 | |
| All* | Total formula weight | 402559.9 |
