6KF1

Microbial Hormone-sensitive lipase- E53 mutant S162A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Lipase	polymer	309	32623.2	3	UniProt (A0A074MDU6) Pfam (PF07859) In PDB	Erythrobacter longus
2	D	Lipase	polymer	310	32738.3	1	UniProt (A0A074MDU6) Pfam (PF07859) In PDB	Erythrobacter longus
3	A, B, C, D	P-NITROPHENOL	non-polymer		139.1	11	Chemie (NPO)
4	A, B, C, D	HEXANOIC ACID	non-polymer		116.2	5	Chemie (6NA)
5	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	C, D	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
8	D	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
9		water	water		18.0	1429	Chemie (HOH)

Sequence modifications

A, B, C: 4 - 312 (UniProt: A0A074MDU6)

PDB	External Database	Details
Ala 162	Ser 162	engineered mutation

D: 3 - 312 (UniProt: A0A074MDU6)

PDB	External Database	Details
Ala 162	Ser 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130607.7
Non-Polymers*	Number of molecules	21
	Total formula weight	2710.6
All*	Total formula weight	133318.3

*Water molecules are not included.