6KF1
Microbial Hormone-sensitive lipase- E53 mutant S162A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-05-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97776 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 70.394, 129.786, 220.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.701 - 1.996 |
Rwork | 0.159 |
R-free | 0.18940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ypv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.738 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.701 | 2.071 |
High resolution limit [Å] | 1.996 | 2.000 |
Rmerge | 0.107 | 0.357 |
Number of reflections | 136953 | 13515 |
<I/σ(I)> | 42.633 | |
Completeness [%] | 99.8 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Calcium chloride, Bis-Tris, PEG MME 550, pH 6.5 |