6KCV
Structure of alginate lyase Aly36B mutant K143A/Y185A in complex with alginate tetrasaccharide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (B, A, C) | Alginate lyase | polymer | 244 | 26836.3 | 3 | UniProt (A0A249T061) | Chitinophaga sp. MD30 | |
| 2 | D, E, F (D, E, F) | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 722.5 | 3 | In PDB GlyTouCan (G72539QT) | |||
| 3 | G, H, I (B, A, C) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 4 | J, K, L (B, A, C) | water | water | 18.0 | 593 | Chemie (HOH) |
Sequence modifications
B, A, C: 19 - 259 (UniProt: A0A249T061)
| PDB | External Database | Details |
|---|---|---|
| Ala 109 | Lys 143 | engineered mutation |
| Ala 151 | Tyr 185 | engineered mutation |
| Leu 260 | - | expression tag |
| Glu 261 | - | expression tag |
| His 262 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 80508.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 2167.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 120.2 | |
| All* | Total formula weight | 82796.7 |
