6KCV

Structure of alginate lyase Aly36B mutant K143A/Y185A in complex with alginate tetrasaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	105.580, 187.526, 91.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.342 - 2.282
R-factor	0.1621
Rwork	0.160
R-free	0.20530
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.770	2.380
High resolution limit [Å]	2.282	2.282
Rmerge	0.120	0.120
Number of reflections	41253	4053
<I/σ(I)>	13.37
Completeness [%]	100.0
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	potassium phosphate dibasic, PEG 3350