6K73

Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Colonization factor antigen I chaperone CfaA	polymer	221	25263.1	2	UniProt (A0A3Y5Z8F9) In PDB	Escherichia coli
2	C, D	CFA/I fimbrial subunit E	polymer	341	37875.5	2	UniProt (P25734) Pfam (PF07434) In PDB	Escherichia coli	Colonization factor antigen I subunit E
3	A, C	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	A, B, C	NICKEL (II) ION	non-polymer		58.7	4	Chemie (NI)
5		water	water		18.0	46	Chemie (HOH)

Sequence modifications

A, B: 0 - 219 (UniProt: A0A3Y5Z8F9)

PDB	External Database	Details
Ile 112	Thr 131	engineered mutation
Ile 114	Leu 133	engineered mutation
Ile 116	Val 135	engineered mutation
Ala 220	-	expression tag

C, D: 24 - 360 (UniProt: P25734)

PDB	External Database	Details
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	126277.2
Non-Polymers*	Number of molecules	8
	Total formula weight	603.1
All*	Total formula weight	126880.3

*Water molecules are not included.