6K73
Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 217.158, 217.158, 177.091 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.517 - 2.774 |
| R-factor | 0.2265 |
| Rwork | 0.225 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.870 |
| High resolution limit [Å] | 2.774 | 2.774 |
| Rmerge | 0.111 | 0.562 |
| Number of reflections | 40388 | 10 |
| <I/σ(I)> | 12.29 | |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2%PEG8000, 0.1M imidazole malate, 1.04M Li2SO4, 0.01M GSSG-GSH |
