6K4P
Crystal structure of xCas9 in complex with sgRNA and DNA (TGG PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | sgRNA | polymer | 84 | 27094.2 | 1 | Streptococcus pyogenes | ||
| 2 | B (C) | DNA (28-MER) | polymer | 28 | 8552.6 | 1 | Streptococcus pyogenes | ||
| 3 | C (D) | non-targeted DNA | polymer | 12 | 3725.5 | 1 | Streptococcus pyogenes | ||
| 4 | D (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158574.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 5 | E, F, G, H, I (B) | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
| 6 | J, K, L, M (A, C, D, B) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Leu 80 | Cys 80 | engineered mutation |
| Thr 262 | Ala 262 | engineered mutation |
| Leu 324 | Arg 324 | engineered mutation |
| Ile 409 | Ser 409 | engineered mutation |
| Lys 480 | Glu 480 | engineered mutation |
| Asp 543 | Glu 543 | engineered mutation |
| Glu 574 | Cys 574 | engineered mutation |
| Ile 694 | Met 694 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| Val 1219 | Glu 1219 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 197947.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 474.9 | |
| All* | Total formula weight | 198421.9 |
