6K41
cryo-EM structure of alpha2BAR-GoA complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 354 | 40100.5 | 1 | UniProt (P09471) Pfam (PF00503) In PDB | Homo sapiens (Human) | |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 349 | 38402.9 | 1 | UniProt (P62874) Pfam (PF00400) In PDB | Mus musculus (Mouse) | Transducin beta chain 1 |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
4 | R | Alpha-2A adrenergic receptor,Endolysin,Alpha-2B adrenergic receptor,Alpha-2B adrenergic receptor | polymer | 512 | 58156.2 | 1 | UniProt (Q28838) UniProt (A0A097J809) UniProt (P18089) Pfam (PF00001) In PDB | Bos taurus (Bovine) | Alpha-2A adrenoreceptor,Alpha-2AAR,Alpha-2D adrenergic receptor,Lysis protein,Lysozyme,Muramidase,Alpha-2 adrenergic receptor subtype C2,Alpha-2B adrenoreceptor,Alpha-2BAR,Alpha-2 adrenergic receptor subtype C2,Alpha-2B adrenoreceptor,Alpha-2BAR |
5 | H | scFv | polymer | 307 | 32898.8 | 1 | Mus musculus | ||
6 | R | 4-[(1~{S})-1-(2,3-dimethylphenyl)ethyl]-1~{H}-imidazole | non-polymer | 200.3 | 1 | Chemie (CZX) |
Sequence modifications
B: 2 - 340 (UniProt: P62874)
R: -187 - -161 (UniProt: Q28838)
R: -153 - 6 (UniProt: A0A097J809)
R: 7 - 354 (UniProt: P18089)
R: 355 - 450 (UniProt: P18089)
PDB | External Database | Details |
---|---|---|
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Asp -198 | - | expression tag |
Asp -197 | - | expression tag |
Asp -196 | - | expression tag |
Asp -195 | - | expression tag |
Ala -194 | - | expression tag |
His -193 | - | expression tag |
His -192 | - | expression tag |
His -191 | - | expression tag |
His -190 | - | expression tag |
His -189 | - | expression tag |
His -188 | - | expression tag |
Glu -160 | - | linker |
Asn -159 | - | linker |
Leu -158 | - | linker |
Tyr -157 | - | linker |
Phe -156 | - | linker |
Gln -155 | - | linker |
Gly -154 | - | linker |
PDB | External Database | Details |
---|---|---|
Thr -101 | Cys 54 | conflict |
Ala -58 | Cys 97 | conflict |
R: 355 - 450 (UniProt: P18089)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 177419.5 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 200.3 | |
All* | Total formula weight | 177619.8 |