6K41

cryo-EM structure of alpha2BAR-GoA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40100.5	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (Human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	349	38402.9	1	UniProt (P62874) Pfam (PF00400)	Mus musculus (Mouse)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (R)	Alpha-2A adrenergic receptor,Endolysin,Alpha-2B adrenergic receptor,Alpha-2B adrenergic receptor	polymer	512	58156.2	1	UniProt (Q28838) UniProt (A0A097J809) UniProt (P18089) Pfam (PF00001)	Bos taurus (Bovine)	Alpha-2A adrenoreceptor,Alpha-2AAR,Alpha-2D adrenergic receptor,Lysis protein,Lysozyme,Muramidase,Alpha-2 adrenergic receptor subtype C2,Alpha-2B adrenoreceptor,Alpha-2BAR,Alpha-2 adrenergic receptor subtype C2,Alpha-2B adrenoreceptor,Alpha-2BAR
5	E (H)	scFv	polymer	307	32898.8	1		Mus musculus
6	F (R)	4-[(1~{S})-1-(2,3-dimethylphenyl)ethyl]-1~{H}-imidazole	non-polymer		200.3	1	Chemie (CZX)

Sequence modifications

B: 2 - 340 (UniProt: P62874)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -187 - -161 (UniProt: Q28838)

PDB	External Database	Details
Asp -198	-	expression tag
Asp -197	-	expression tag
Asp -196	-	expression tag
Asp -195	-	expression tag
Ala -194	-	expression tag
His -193	-	expression tag
His -192	-	expression tag
His -191	-	expression tag
His -190	-	expression tag
His -189	-	expression tag
His -188	-	expression tag
Glu -160	-	linker
Asn -159	-	linker
Leu -158	-	linker
Tyr -157	-	linker
Phe -156	-	linker
Gln -155	-	linker
Gly -154	-	linker

R: -153 - 6 (UniProt: A0A097J809)

PDB	External Database	Details
Thr -101	Cys 54	conflict
Ala -58	Cys 97	conflict

R: 7 - 354 (UniProt: P18089)
R: 355 - 450 (UniProt: P18089)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	177419.5
Non-Polymers*	Number of molecules	1
	Total formula weight	200.3
All*	Total formula weight	177619.8

*Water molecules are not included.