6K37

Crystal structure of BioU (K124A) from Synechocystis sp.PCC6803 in complex with NAD+ and the analog of reaction intermediate, 3-(1-aminoethyl)-nonanedioic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Slr0355 protein	polymer	353	37794.8	1	UniProt (Q55650) Pfam (PF00208) In PDB	Synechocystis sp. PCC 6803	BioU
2	A	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	1	Chemie (NAD)
3	A	(3R)-3-[(1R)-1-azanylethyl]nonanedioic acid	non-polymer		231.3	1	Chemie (CWL)
4		water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 1 - 331 (UniProt: Q55650)

PDB	External Database	Details
Met -21	-	expression tag
Lys -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 124	Lys 124	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37794.8
Non-Polymers*	Number of molecules	2
	Total formula weight	894.7
All*	Total formula weight	38689.5

*Water molecules are not included.