6JUQ

mutant PolIV-DNA incoming nucleotide complex 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F	DNA polymerase IV	polymer	342	38346.4	1	UniProt (W8STT9) Pfam (PF00817) Pfam (PF21999) Pfam (PF11799) UniProt (by SIFTS) (Q47155) In PDB	Escherichia coli	Pol IV
2	G, B	DNA (5'-D(P*CP*TP*GP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	17	5204.4	2		Escherichia coli
3	H	DNA (5'-D(P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	14	4281.8	1		Escherichia coli
4	A	DNA polymerase IV	polymer	341	38289.4	1	UniProt (W8STT9) Pfam (PF00817) Pfam (PF21999) Pfam (PF11799) UniProt (by SIFTS) (Q47155) In PDB	Escherichia coli	Pol IV
5	C	DNA (5'-D(P*GP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3')	polymer	15	4611.0	1		Escherichia coli
6	F, A	5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine	non-polymer		481.2	2	Chemie (2TM)
7	F, A	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
8		water	water		18.0	14	Chemie (HOH)

Sequence modifications

F: 2 - 341 (UniProt: W8STT9)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 13	Phe 13	engineered mutation
Cys 43	Thr 43	engineered mutation

A: 2 - 341 (UniProt: W8STT9)

PDB	External Database	Details
Ser 1	-	expression tag
Ala 13	Phe 13	engineered mutation
Cys 43	Thr 43	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	95937.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1182.1
All*	Total formula weight	97119.4

*Water molecules are not included.