6JUQ
mutant PolIV-DNA incoming nucleotide complex 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.151, 57.244, 110.793 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 86.010 - 2.740 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.27084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4irc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.665 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.010 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.104 | |
| Number of reflections | 28806 | 4105 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.997 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 277 | MDP 1%, Phosphate buffer ph 4.7 |
