6JR8

Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Candidate alpha-glycosidase Glycoside hydrolase family 31	polymer	838	96138.5	4	UniProt (A5FBI1) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) Pfam (PF17137)	Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)	Dextranase
2	E, F, G, H (E, F, G, H)	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		504.4	4	In PDB GlyTouCan (G02998EX)
3	I, L, Q, U (A, B, C, D)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
4	J, K, M, N, O... (A, B, C, D)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	13	Chemie (MPD)
5	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	3621	Chemie (HOH)

Sequence modifications

A, B, C, D: 21 - 836 (UniProt: A5FBI1)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Pro 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
Met 19	-	expression tag
Ala 20	-	expression tag
Ala 412	Asp 412	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	384553.9
Branched	Number of molecules	4
	Total formula weight	2017.8
Non-Polymers*	Number of molecules	17
	Total formula weight	1772.4
All*	Total formula weight	388344.1

*Water molecules are not included.