6JR8
Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 102.370, 112.625, 114.172 |
Unit cell angles | 111.06, 92.98, 114.54 |
Refinement procedure
Resolution | 47.380 - 1.800 |
R-factor | 0.1512 |
Rwork | 0.150 |
R-free | 0.17360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jr6 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.780 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.054 | 0.400 |
Number of reflections | 378141 | 18689 |
<I/σ(I)> | 15.3 | 2 |
Completeness [%] | 96.9 | 95.4 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM sodium acetate buffer, 8% (w/v) polyethylene glycol 20000, and 8% 2-methyl-2,4-pentanediol |