6JOE

Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA (guanine-N(1)-)-methyltransferase	polymer	269	30336.2	2	UniProt (Q02RL6) Pfam (PF01746)	Pseudomonas aeruginosa UCBPP-PA14	M1G-methyltransferase,tRNA [GM37] methyltransferase
2	C (A)	~{N}-[[4-[(4-azanylpiperidin-1-yl)methyl]phenyl]methyl]-4-oxidanylidene-3~{H}-thieno[2,3-d]pyrimidine-5-carboxamide	non-polymer		397.5	1	Chemie (BWR)
3	D (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	E (B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5	F, G (A, B)	water	water		18.0	191	Chemie (HOH)

Sequence modifications

A, B: 5 - 250 (UniProt: Q02RL6)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Val -8	-	expression tag
Asp -7	-	expression tag
Leu -6	-	expression tag
Gly -5	-	expression tag
Thr -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60672.3
Non-Polymers*	Number of molecules	3
	Total formula weight	890.9
All*	Total formula weight	61563.2

*Water molecules are not included.