6JDV
Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in catalytic state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1083 | 124633.9 | 1 | UniProt (C9X1G5) Pfam (PF13395) Pfam (PF18541) | Neisseria meningitidis serogroup C (strain 8013) | |
| 2 | B (B) | sgRNA | polymer | 147 | 46901.6 | 1 | synthetic construct | ||
| 3 | C (C) | target DNA strand | polymer | 35 | 10836.0 | 1 | synthetic construct | ||
| 4 | D (D) | non-target DNA strand | polymer | 11 | 3362.2 | 1 | synthetic construct | ||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 7 | G, H (A, B) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A: 1 - 1082 (UniProt: C9X1G5)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 588 | His 588 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 185733.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 86.4 | |
| All* | Total formula weight | 185820.2 |
