6JCY

Mycobacterium tuberculosis RNA polymerase transcription initiation open complex with a chimeric ECF sigma factor sigH/E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	368	40279.0	2	UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1174	129602.3	1	UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1317	147068.1	1	UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5) Pfam (PF01192) In PDB	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	ECF RNA polymerase sigma factor SigH,ECF RNA polymerase sigma factor SigE,ECF RNA polymerase sigma factor SigH	polymer	209	23561.4	1	UniProt (P9WGH9) UniProt (P9WGG7) Pfam (PF04542) Pfam (PF08281) In PDB	Mycobacterium tuberculosis H37Rv	ECF sigma factor SigH,Alternative RNA polymerase sigma factor SigH,RNA polymerase sigma-H factor,Sigma-H factor,ECF sigma factor SigE,Alternative RNA polymerase sigma factor SigE,RNA polymerase sigma-E factor,Sigma-E factor
6	G	DNA (5'-D(*TP*TP*GP*TP*GP*GP*GP*AP*GP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*CP*A)-3')	polymer	23	7152.6	1		synthetic construct
7	H	DNA (5'-D(*TP*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*TP*CP*GP*AP*GP*GP*GP*T)-3')	polymer	20	6206.0	1		synthetic construct
8	I	RNA (5'-R(*CP*CP*UP*CP*GP*A)-3')	polymer	6	1851.2	1		synthetic construct
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11		water	water		18.0	130	Chemie (HOH)

Sequence modifications

A, B: 1 - 347 (UniProt: P9WGZ1)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag

C: 7 - 1178 (UniProt: P9WGY9)

PDB	External Database	Details
Met 5	-	initiating methionine
Val 6	-	expression tag

D: 2 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag

F: 1 - 95 (UniProt: P9WGH9)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

F: 96 - 134 (UniProt: P9WGG7)
F: 135 - 207 (UniProt: P9WGH9)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	407850.8
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	408005.9

*Water molecules are not included.