6JCY
Mycobacterium tuberculosis RNA polymerase transcription initiation open complex with a chimeric ECF sigma factor sigH/E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97916 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 128.943, 163.005, 129.315 |
Unit cell angles | 90.00, 117.81, 90.00 |
Refinement procedure
Resolution | 48.766 - 3.106 |
R-factor | 0.1959 |
Rwork | 0.195 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zx3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.090 | 0.039 | 0.871 |
Rmeas | 0.102 | 0.045 | 0.990 |
Rpim | 0.047 | 0.020 | 0.458 |
Number of reflections | 78903 | 4028 | 2980 |
<I/σ(I)> | 12.3 | 1.59 | |
Completeness [%] | 93.3 | 93 | 70.9 |
Redundancy | 4.2 | 4.1 | 4 |
CC(1/2) | 0.995 | 0.996 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M sodium acetate, 0.1M sodium citrate pH 5.5, 10% PEG4000 |