6JB0

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with trehalose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ABC transporter, periplasmic substrate-binding protein	polymer	408	46052.9	1	UniProt (Q5SLD7) Pfam (PF01547) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	alpha-GlyBP
2	B	alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900006) GlyTouCan (G92130SN)		trehalose
3	A	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	A	1,3-PROPANDIOL	non-polymer		76.1	1	Chemie (PDO)
7		water	water		18.0	620	Chemie (HOH)

Sequence modifications

A: 1 - 402 (UniProt: Q5SLD7)

PDB	External Database	Details
Ala 287	Trp 314	engineered mutation
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46052.9
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	5
	Total formula weight	484.4
All*	Total formula weight	46879.6

*Water molecules are not included.