6JB0

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.760, 84.760, 146.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.330 - 1.630
R-factor	0.1355
Rwork	0.134
R-free	0.16020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.020
RMSD bond angle	1.947
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.330	8.920
High resolution limit [Å]	1.630	1.630
Rmerge	0.061	0.381
Rmeas	0.066	0.414
Rpim	0.025	0.160
Number of reflections	65184	2980
<I/σ(I)>	24.4	5.7
Completeness [%]	96.9	91.5
Redundancy	12.3	11.9
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350