6JAL

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in ligand free form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ABC transporter, periplasmic substrate-binding protein	polymer	416	46980.0	1	UniProt (Q5SLD7) Pfam (PF01547)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	alpha-GlyBP
2	B, C (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
3	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	E, F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	G, H (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	I (A)	water	water		18.0	666	Chemie (HOH)

Sequence modifications

A: 1 - 402 (UniProt: Q5SLD7)

PDB	External Database	Details
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Met -1	-	expression tag
Met 0	-	expression tag
Ala 356	Arg 383	engineered mutation
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46980.0
Non-Polymers*	Number of molecules	7
	Total formula weight	546.6
All*	Total formula weight	47526.5

*Water molecules are not included.