6JAL
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in ligand free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2018-02-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.040, 85.130, 55.340 |
Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
Resolution | 55.260 - 1.560 |
R-factor | 0.1282 |
Rwork | 0.127 |
R-free | 0.15050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j9w |
RMSD bond length | 0.022 |
RMSD bond angle | 2.155 |
Data reduction software | iMOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.260 | 8.540 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.031 | 0.061 |
Rmeas | 0.040 | 0.077 |
Rpim | 0.024 | 0.048 |
Number of reflections | 69683 | 3351 |
<I/σ(I)> | 30.1 | 15 |
Completeness [%] | 99.8 | 96.4 |
Redundancy | 4.6 | 4.2 |
CC(1/2) | 0.999 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5 | 277 | 0.05 M Citric Acid, 0.05 Bis-Tris Propane, 16% PEG 3350 |