6JAL

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in ligand free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	53.040, 85.130, 55.340
Unit cell angles	90.00, 92.99, 90.00

Refinement procedure

Resolution	55.260 - 1.560
R-factor	0.1282
Rwork	0.127
R-free	0.15050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.022
RMSD bond angle	2.155
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.260	8.540
High resolution limit [Å]	1.560	1.560
Rmerge	0.031	0.061
Rmeas	0.040	0.077
Rpim	0.024	0.048
Number of reflections	69683	3351
<I/σ(I)>	30.1	15
Completeness [%]	99.8	96.4
Redundancy	4.6	4.2
CC(1/2)	0.999	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	277	0.05 M Citric Acid, 0.05 Bis-Tris Propane, 16% PEG 3350