6J7A

Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (17aa)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Heme oxygenase 1,NADPH--cytochrome P450 reductase	polymer	875	99886.7	2	UniProt (P06762) UniProt (P00388) Pfam (PF01126) Pfam (PF00258) Pfam (PF00667) Pfam (PF00175)	Rattus norvegicus (Rat)	HO-1,HSP32,P450R
2	C, F (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	D, G (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	E, H (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
5	I, J (A, B)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A, B: 1 - 237 (UniProt: P06762)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Pro 222	Thr 222	engineered mutation
Ala 230	Pro 230	engineered mutation
Met 238	-	linker

A, B: 239 - 855 (UniProt: P00388)

PDB	External Database	Details
-	Thr 236	deletion
-	Gly 237	deletion
-	Glu 238	deletion
-	Glu 239	deletion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	199773.4
Non-Polymers*	Number of molecules	6
	Total formula weight	3716.8
All*	Total formula weight	203490.2

*Water molecules are not included.