6J7A
Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (17aa)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| A | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| A | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| A | 0004392 | molecular_function | heme oxygenase (decyclizing) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006788 | biological_process | heme oxidation |
| A | 0007584 | biological_process | response to nutrient |
| A | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009437 | biological_process | carnitine metabolic process |
| A | 0009725 | biological_process | response to hormone |
| A | 0009812 | biological_process | flavonoid metabolic process |
| A | 0010181 | molecular_function | FMN binding |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019395 | biological_process | fatty acid oxidation |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0022900 | biological_process | electron transport chain |
| A | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| A | 0043066 | biological_process | negative regulation of apoptotic process |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0043602 | biological_process | nitrate catabolic process |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| A | 0046210 | biological_process | nitric oxide catabolic process |
| A | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070988 | biological_process | demethylation |
| A | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| A | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| A | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| A | 0071548 | biological_process | response to dexamethasone |
| A | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| A | 0090181 | biological_process | regulation of cholesterol metabolic process |
| A | 0090346 | biological_process | cellular organofluorine metabolic process |
| B | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| B | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| B | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| B | 0004392 | molecular_function | heme oxygenase (decyclizing) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0006788 | biological_process | heme oxidation |
| B | 0007584 | biological_process | response to nutrient |
| B | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0009437 | biological_process | carnitine metabolic process |
| B | 0009725 | biological_process | response to hormone |
| B | 0009812 | biological_process | flavonoid metabolic process |
| B | 0010181 | molecular_function | FMN binding |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019395 | biological_process | fatty acid oxidation |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0022900 | biological_process | electron transport chain |
| B | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| B | 0043066 | biological_process | negative regulation of apoptotic process |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0043602 | biological_process | nitrate catabolic process |
| B | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| B | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| B | 0046210 | biological_process | nitric oxide catabolic process |
| B | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070988 | biological_process | demethylation |
| B | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| B | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| B | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| B | 0071548 | biological_process | response to dexamethasone |
| B | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| B | 0090181 | biological_process | regulation of cholesterol metabolic process |
| B | 0090346 | biological_process | cellular organofluorine metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | binding site for residue HEM A 901 |
| Chain | Residue |
| A | LYS18 |
| A | ARG183 |
| A | PHE207 |
| A | HIS25 |
| A | GLU29 |
| A | TYR134 |
| A | THR135 |
| A | ARG136 |
| A | GLY139 |
| A | SER142 |
| A | LYS179 |
| site_id | AC2 |
| Number of Residues | 16 |
| Details | binding site for residue FAD A 902 |
| Chain | Residue |
| A | HIS496 |
| A | ARG601 |
| A | ARG631 |
| A | TYR632 |
| A | TYR633 |
| A | SER634 |
| A | CYS649 |
| A | ALA650 |
| A | VAL651 |
| A | VAL653 |
| A | TYR655 |
| A | GLY665 |
| A | VAL666 |
| A | ALA667 |
| A | THR668 |
| A | TRP854 |
| site_id | AC3 |
| Number of Residues | 19 |
| Details | binding site for residue FMN A 903 |
| Chain | Residue |
| A | SER267 |
| A | GLN268 |
| A | THR269 |
| A | GLY270 |
| A | THR271 |
| A | ALA272 |
| A | ALA319 |
| A | THR320 |
| A | TYR321 |
| A | GLY322 |
| A | LEU354 |
| A | GLY355 |
| A | ASN356 |
| A | TYR359 |
| A | HIS361 |
| A | PHE362 |
| A | ASN363 |
| A | ASP389 |
| A | LEU393 |
| site_id | AC4 |
| Number of Residues | 12 |
| Details | binding site for residue HEM B 901 |
| Chain | Residue |
| B | LYS18 |
| B | HIS25 |
| B | GLU29 |
| B | GLN38 |
| B | TYR134 |
| B | THR135 |
| B | ARG136 |
| B | GLY139 |
| B | SER142 |
| B | LYS179 |
| B | ARG183 |
| B | PHE207 |
| site_id | AC5 |
| Number of Residues | 17 |
| Details | binding site for residue FAD B 902 |
| Chain | Residue |
| B | HIS496 |
| B | ARG601 |
| B | ARG631 |
| B | TYR632 |
| B | TYR633 |
| B | SER634 |
| B | CYS649 |
| B | ALA650 |
| B | VAL651 |
| B | VAL653 |
| B | TYR655 |
| B | GLY665 |
| B | VAL666 |
| B | ALA667 |
| B | THR668 |
| B | TRP854 |
| B | SER855 |
| site_id | AC6 |
| Number of Residues | 19 |
| Details | binding site for residue FMN B 903 |
| Chain | Residue |
| B | SER267 |
| B | GLN268 |
| B | THR269 |
| B | GLY270 |
| B | THR271 |
| B | ALA272 |
| B | ALA319 |
| B | THR320 |
| B | TYR321 |
| B | GLY322 |
| B | LEU354 |
| B | GLY355 |
| B | ASN356 |
| B | TYR359 |
| B | HIS361 |
| B | PHE362 |
| B | ASN363 |
| B | ASP389 |
| B | LEU393 |
Functional Information from PROSITE/UniProt
| site_id | PS00593 |
| Number of Residues | 11 |
| Details | HEME_OXYGENASE Heme oxygenase signature. LVAHAYTRYLG |
| Chain | Residue | Details |
| A | LEU129-GLY139 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | LYS18 | |
| A | TYR134 | |
| A | ARG183 | |
| B | LYS18 | |
| B | TYR134 | |
| B | ARG183 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: axial binding residue => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | HIS25 | |
| B | HIS25 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Important for catalytic activity => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | ASP140 | |
| B | ASP140 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | SER229 | |
| B | SER229 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | SER267 | |
| B | SER267 | |
| B | ALA319 | |
| B | LEU354 | |
| B | ILE635 | |
| B | SER659 | |
| B | SER669 | |
| B | PRO716 | |
| B | ALA777 | |
| B | GLN783 | |
| A | ALA319 | |
| A | LEU354 | |
| A | ILE635 | |
| A | SER659 | |
| A | SER669 | |
| A | PRO716 | |
| A | ALA777 | |
| A | GLN783 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| A | ASP389 | |
| B | ASP389 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| A | HIS479 | |
| A | THR820 | |
| B | HIS479 | |
| B | THR820 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | LEU605 | |
| B | LEU605 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | VAL653 | |
| B | VAL653 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| A | SER638 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| A | ARG811 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| B | SER638 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| B | ARG811 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
