6J4H
Crystal Structure of maltotriose-complex of PulA-G680L mutant from Klebsiella pneumoniae
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Pullulanase | polymer | 1053 | 114518.8 | 1 | UniProt (W9BQ28) Pfam (PF03714) Pfam (PF17967) Pfam (PF02922) Pfam (PF18494) Pfam (PF11852) In PDB | Klebsiella pneumoniae | PulA protein,Pullulanase-type alpha-1,6-glucosidase,DUF3372 domain-containing protein |
| 2 | B, C | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 2 | In PDB BIRD (PRD_900009) GlyTouCan (G96370VA) | alpha-maltotriose | ||
| 3 | A | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | water | water | 18.0 | 801 | Chemie (HOH) |
Sequence modifications
A: 32 - 1083 (UniProt: W9BQ28)
| PDB | External Database | Details |
|---|---|---|
| Met 31 | - | initiating methionine |
| Ser 229 | Asn 248 | see sequence details |
| Leu 680 | Gly 699 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 114518.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1008.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 405.0 | |
| All* | Total formula weight | 115932.7 |
