6J4H
Crystal Structure of maltotriose-complex of PulA-G680L mutant from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.943, 60.103, 133.962 |
| Unit cell angles | 90.00, 114.66, 90.00 |
Refinement procedure
| Resolution | 38.247 - 1.641 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.18370 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.804 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.641 | 1.641 |
| Rmerge | 0.069 | 0.339 |
| Rmeas | 0.081 | 0.399 |
| Rpim | 0.041 | 0.209 |
| Number of reflections | 131547 | 12890 |
| <I/σ(I)> | 16.9 | 3.6 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 3.8 | 3.5 |
| CC(1/2) | 0.997 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium acetate buffer (pH 6.0), 0.2M magnesium acetate, 20%(w/v) PEG 6000 |
