6J28
Crystal structure of the branched-chain polyamine synthase C9 mutein from Thermus thermophilus (Tth-BpsA C9) in complex with N4-aminopropylspermidine and 5'-methylthioadenosine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | N(4)-bis(aminopropyl)spermidine synthase | polymer | 375 | 41734.3 | 4 | UniProt (Q72L89) Pfam (PF01861) | Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) | Branched-chain polyamine synthase A |
| 2 | E, H, L, O (A, B, C, D) | N,N-bis(3-aminopropyl)butane-1,4-diamine | non-polymer | 202.3 | 4 | Chemie (N4P) | |||
| 3 | F, I, M, P (A, B, C, D) | 5'-DEOXY-5'-METHYLTHIOADENOSINE | non-polymer | 297.3 | 4 | Chemie (MTA) | |||
| 4 | G, J (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
| 5 | K, N, Q (B, C, D) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 3 | Chemie (MES) | |||
| 6 | R, S, T, U (A, B, C, D) | water | water | 18.0 | 499 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 354 (UniProt: Q72L89)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Val 1 | - | insertion |
| Tyr 346 | - | See sequence details |
| Asp 347 | - | See sequence details |
| Asp 348 | - | See sequence details |
| Glu 349 | - | See sequence details |
| Glu 350 | - | See sequence details |
| Ser 351 | - | See sequence details |
| Ser 352 | - | See sequence details |
| Thr 353 | - | See sequence details |
| Thr 354 | - | See sequence details |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 166937.4 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2696.1 | |
| All* | Total formula weight | 169633.5 |
