6IV8
the selenomethionine(SeMet)-derived Cas13d binary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | The selenomethionine (SeMet)-labeled Cas13d | polymer | 930 | 108759.3 | 2 | UniProt (A0A1C5SD84) | uncultured Ruminococcus sp | |
| 2 | B (B) | RNA (51-MER) | polymer | 51 | 16244.7 | 1 | uncultured Ruminococcus sp. | ||
| 3 | C (D) | RNA (53-MER) | polymer | 53 | 16879.1 | 1 | uncultured Ruminococcus sp. | ||
| 4 | E, F (B, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | G, H, I, J (A, B, D, C) | water | water | 18.0 | 665 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 922 (UniProt: A0A1C5SD84)
| PDB | External Database | Details |
|---|---|---|
| Ala 288 | Arg 288 | engineered mutation |
| Ala 823 | Arg 823 | engineered mutation |
| Leu 923 | - | expression tag |
| Glu 924 | - | expression tag |
| His 925 | - | expression tag |
| His 926 | - | expression tag |
| His 927 | - | expression tag |
| His 928 | - | expression tag |
| His 929 | - | expression tag |
| His 930 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 250642.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 250691.1 |
