6IV8

the selenomethionine(SeMet)-derived Cas13d binary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	The selenomethionine (SeMet)-labeled Cas13d	polymer	930	108759.3	2	UniProt (A0A1C5SD84) In PDB	uncultured Ruminococcus sp
2	B	RNA (51-MER)	polymer	51	16244.7	1		uncultured Ruminococcus sp.
3	D	RNA (53-MER)	polymer	53	16879.1	1		uncultured Ruminococcus sp.
4	B, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	665	Chemie (HOH)

Sequence modifications

A, C: 1 - 922 (UniProt: A0A1C5SD84)

PDB	External Database	Details
Ala 288	Arg 288	engineered mutation
Ala 823	Arg 823	engineered mutation
Leu 923	-	expression tag
Glu 924	-	expression tag
His 925	-	expression tag
His 926	-	expression tag
His 927	-	expression tag
His 928	-	expression tag
His 929	-	expression tag
His 930	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	250642.5
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	250691.1

*Water molecules are not included.