6IV8
the selenomethionine(SeMet)-derived Cas13d binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.164, 145.733, 249.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.920 - 2.150 |
| R-factor | 0.1898 |
| Rwork | 0.189 |
| R-free | 0.21030 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.781 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.920 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.129 | |
| Rpim | 0.053 | 0.337 |
| Number of reflections | 129626 | 6409 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.3 | |
| CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | (0.5% (w/v) Tryptone, 0.1 M HEPES sodium (pH 7.0), 16% (w/v) Polyethylene glycol 3350) |
