6IQS

Crystal structure of Prc with L245A and L340G mutations in complex with NlpI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lipoprotein NlpI	polymer	296	33841.6	2	UniProt (P0AFB1)	Escherichia coli
2	C, D (C, D)	Tail-specific protease	polymer	688	77496.7	2	UniProt (P23865) Pfam (PF17804) Pfam (PF00595) Pfam (PF03572) Pfam (PF11818)	Escherichia coli (strain K12)	C-terminal-processing peptidase,PRC protein,Protease Re
3	E, F, G, H (A, B, C, D)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B: 22 - 296 (UniProt: P0AFB1)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag

C, D: 1 - 682 (UniProt: P23865)

PDB	External Database	Details
Ala 245	Leu 245	engineered mutation
Gly 340	Leu 340	engineered mutation
His 683	-	expression tag
His 684	-	expression tag
His 685	-	expression tag
His 686	-	expression tag
His 687	-	expression tag
His 688	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	222676.5
All*	Total formula weight	222676.5

*Water molecules are not included.