6IQS
Crystal structure of Prc with L245A and L340G mutations in complex with NlpI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-27 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.10000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 119.342, 144.415, 152.504 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.910 - 2.690 |
R-factor | 0.20695 |
Rwork | 0.205 |
R-free | 0.24522 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wql |
RMSD bond length | 0.016 |
RMSD bond angle | 2.041 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 (v712) |
Phasing software | PHASER (2.70) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.064 | 0.548 |
Rmeas | 0.098 | 0.813 |
Rpim | 0.042 | 0.394 |
Number of reflections | 69840 | 6187 |
<I/σ(I)> | 13.47 | 3.684 |
Completeness [%] | 94.7 | 85.1 |
Redundancy | 5 | 3.5 |
CC(1/2) | 0.998 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG3350, Sodium citrate tribasic dihydrate |