6IID

Human EXOG-H140A in complex with RNA-DNA chimeric duplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Nuclease EXOG, mitochondrial	polymer	348	39216.3	4	UniProt (Q9Y2C4) Pfam (PF01223) Pfam (PF18026)	Homo sapiens (Human)	Endonuclease G-like 1,Endo G-like 1
2	E, G, I, K (E, G, I, K)	DNA/RNA (5'-R(P*CP*GP*GP*GP*A)-D(P*T)-R(P*G)-D(P*T)-R(P*CP*AP*CP*G)-3')	polymer	12	3735.4	4		synthetic construct	R2-DNA
3	F, H, J, L (F, H, J, L)	DNA (5'-D(*CP*GP*TP*GP*AP*CP*AP*TP*CP*CP*CP*G)-3')	polymer	12	3623.4	4		synthetic construct
4	M, N, O, P (B, C, E, K)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	44	Chemie (HOH)

Sequence modifications

A, B, C, D: 42 - 368 (UniProt: Q9Y2C4)

PDB	External Database	Details
Met 21	-	expression tag
Gly 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Ser 31	-	expression tag
Ser 32	-	expression tag
Gly 33	-	expression tag
Leu 34	-	expression tag
Val 35	-	expression tag
Pro 36	-	expression tag
Arg 37	-	expression tag
Gly 38	-	expression tag
Ser 39	-	expression tag
His 40	-	expression tag
Met 41	-	expression tag
Ala 140	His 140	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	186300.2
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	186397.4

*Water molecules are not included.