6II6

Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Putative RTX-toxin	polymer	371	40821.3	2	Pfam (PF03543) UniProt (by SIFTS) (F1CLG9) In PDB	Vibrio vulnificus
2	C, D	ADP-ribosylation factor 3	polymer	172	19796.6	2	UniProt (P61204) Pfam (PF00025) In PDB	Homo sapiens (Human)
3	C, D	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
4	C, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	581	Chemie (HOH)

Sequence modifications

C, D: 14 - 176 (UniProt: P61204)

PDB	External Database	Details
Met 13	-	initiating methionine
Leu 71	Gln 71	engineered mutation
Leu 177	-	expression tag
Glu 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	121235.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	122330.7

*Water molecules are not included.