6IDF
Cryo-EM structure of gamma secretase in complex with a Notch fragment
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Nicastrin | polymer | 709 | 78483.6 | 1 | UniProt (Q92542) Pfam (PF18266) Pfam (PF05450) In PDB | Homo sapiens (Human) | |
2 | B | Presenilin-1 | polymer | 467 | 52644.5 | 1 | UniProt (P49768) Pfam (PF01080) In PDB | Homo sapiens (Human) | PS-1 |
3 | C | Gamma-secretase subunit APH-1A | polymer | 265 | 29017.9 | 1 | UniProt (Q96BI3) Pfam (PF06105) In PDB | Homo sapiens (Human) | APH-1a |
4 | D | Gamma-secretase subunit PEN-2 | polymer | 101 | 12038.0 | 1 | UniProt (Q9NZ42) Pfam (PF10251) In PDB | Homo sapiens (Human) | Presenilin enhancer protein 2 |
5 | E | Notch1 | polymer | 129 | 14758.5 | 1 | UniProt (by SIFTS) (P46531) In PDB | Homo sapiens | |
6 | F, H, I, J, K | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
7 | G | beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G89225LT) | |||
8 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
9 | B, C, D | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 4 | Chemie (PC1) | |||
10 | C | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) |
Sequence modifications
B: 1 - 467 (UniProt: P49768)
PDB | External Database | Details |
---|---|---|
Cys 112 | Gln 112 | engineered mutation |
Ala 385 | Asp 385 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 186942.6 | |
Branched | Number of molecules | 6 |
Total formula weight | 3032.8 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 5647.8 | |
All* | Total formula weight | 195623.2 |