6IB5

Mutant of flavin-dependent tryptophan halogenase Thal with altered regioselectivity (Thal-RebH5)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Tryptophan 6-halogenase	polymer	534	60376.1	2	UniProt (A1E280) Pfam (PF04820) In PDB	Streptomyces albogriseolus
2	A, B	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	A, B	GLYCEROL	non-polymer		92.1	11	Chemie (GOL)
4		water	water		18.0	420	Chemie (HOH)

Sequence modifications

A, B: 2 - 531 (UniProt: A1E280)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Ile 52	Val 52	engineered mutation
Ile 82	Val 82	engineered mutation
Thr 360	Ser 360	engineered mutation
Ser 469	Gly 469	engineered mutation
Asn 470	Ser 470	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120752.2
Non-Polymers*	Number of molecules	15
	Total formula weight	1392.9
All*	Total formula weight	122145.2

*Water molecules are not included.